Non-Surgical Model of Sepsis and Inflammation
In this model, a cecal slurry is used to induce septic peritonitis resulting in systemic bacteremia, organ infection and eventual systemic release of cytokines. The model is highly reproducible, easy to monitor by a variety of endpoints, and closely mimics clinical sepsis. By titrating the amount of cecal slurry used, the severity of the septic response can be adjusted to the needs of the experimenter.
- The model is a robust and reproducible preclinical model of poly-microbial sepsis to facilitate drug screening. It can be used in a variety of different types of studies including development of antibiotics, antithrombotics, immune modifiers, and diagnostics.
Groups of 8 mice each received intraperitoneal (IP) injections at doses ranging from 0.5 gm/kg to 4 gm/kg.
Animals were monitored every hour and deaths recorded. Survival was dose dependent.
- Because infection is poly-microbial, involving both gram negative and gram positive bacteria, a wide range of pattern recognition receptors such as Toll-Like Receptors are activated on a variety of immune cells, mimicking sepsis.
- The model is useful for diagnostic and therapeutic studies of sepsis induced by pathogens, sterile sepsis or by trauma as the inflammatory response and Multi-Organ Dysfunction Syndrome (MODS) characteristic of Systemic Inflammatory Response Syndrome (SIRS) is similar whether induced by inflammation or by trauma.