Human Xenograft Models
Mouse models of cancer have consistently been used to determine the in vivo activity of new anti-cancer therapeutics prior to clinical development and testing in humans. The most common models are xenografts of human tumors and cell lines grown subcutaneously in immunodeficient mice such as athymic (nude) or severe combined immune deficient (SCID) mice. These mouse strains exhibit very high take rates for xenografts, making them ideal hosts for in vivo propagation of human tumor cells.
- Xenograft models are commonly used to determine ideal drug dosing, treatment schedules, and routes of drug administration that maximize anti-tumor efficacy and therapeutic window.
Human ASPC-1 human pancreatic cancer cells were injected subcutaneously into the right flank of 8 week old female NU NU nude mice (Crl:NU-Foxn1nu). Tumor size measurements were initiated 8 days post inoculation and monitored three times per week. Tumor measurements were evaluated using calipers;tumor size was calculated using the following formula: (Length x width2)/2. Data are plotted as mean tumor volume +/- standard error of the mean.